Biochemistry I
FangYi易芳
目录
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1 Introduction
1.1 What is biochemistry
1.2 Why and how
1.3 Review of some important terms in chemistry
2 Protein
2.1 Chemical components of proteins
2.2 Amino Acids
2.3 Molecular Structures of Proteins
2.4 Biological Functions of Proteins
2.5 Structure-Function Relationship of Proteins
2.6 Physical and Chemical Properties
2.7 Working with proteins
2.8 Review: noncovalent interations
3 Enzyme
3.1 Introduction of enzymes
3.2 How enzymes work
3.3 Regulation of enzymes
3.4 Clinical applications of enzymes
4 Vitamins
4.1 Fat soluble vitamins
4.2 Water soluble vitamins
5 Nucleotides and Nucleic Acids
5.1 Nucleotide
5.2 Structure and Function of DNA
5.3 Structure and Function of RNA
5.4 Chemical Properties of Nucleic Acids
6 DNA replication and repair
6.1 General concepts of replication
6.2 Enzymology of DNA synthesis
6.3 Replication processes
6.4 Other DNA replication mode
6.5 DNA damage and repair
7 Transcription
7.1 Template and enzymes
7.2 Transcription process
7.3 Post-transcriptional modification
8 Translation
8.1 Protein Synthetic System
8.2 Protein Synthetic Process
8.3 Protein Modification and Targeting
8.4 Clinical Correlation of Translation
9 Regulation of Gene Expression
9.1 08-1 Principles and concepts
9.2 08-2 Gene Regulation of Prokaryotic Systems
9.3 08-3 Regulation of Eukaryotic systems
10 Recombinant DNA Technology
10.1 Introduction of Recombinant DNA Technology
11 Experiments
11.1 Use of Pipet and Buret
11.2 Use of Micropipettes
11.3 Protein Salt Precipitation and Dialysis
11.4 Determination of Vitamin C Content in Fruits
11.5 LDH
11.6 Detection of Ketone Bodies
11.7 Determination of Serum Protein Concentration
11.8 Agarose Gel Electrophoresis
11.9 Dialysis tube
12 Review
12.1 Review of Biochemistry I
Physical and Chemical Properties
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