研究领域

CRISPR/Cas9基因敲除技术; CRISPR/dCas9基因抑制; CRISPR/dCas9-AID/TadA碱基编辑技术; 基因调控；Golden Gate质粒构建技术; 基因编辑；重组蛋白的过表达与纯化; 微生物电合成平台的搭建; 多gRNA质粒的构建; RNA的提取; SDS-PAGE; 蛋白质谱分析技术; 重组质粒构建; 核磁代谢组学; 微生物代谢工程与合成生物学技术; HPLC；RT-qPCR；发酵过程优化；；基本的动物细胞培养与实验操作;基本的小鼠实验操作; MTT Assay; Scratch test; Transwell (Migration Test and Invasion Test); Plate colony formation assay.

论文与著作

1、Zaiqiang Wu, Dongdong Zhao, Siwei Li, Junsong Wang*, Changhao Bi*, Xueli Zhang*. Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli. MicrobiologyOpen, 2019, 8(12): e930. https://doi.org/10.1002/mbo3.930, 中科院三区；JCR二区；IF = 3.904; Cited by 9;

2、Zaiqiang Wu, Junsong Wang*, Jun Liu, Yan Wang, Changhao Bi*, Xueli Zhang*. Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO₂. Microbial Cell Factories, 2019, 18(1):15, https://doi.org/10.1186/s12934-019-1067-3, 中科院二区；JCR一区；IF = 6.352; Cited by 60;

3、Zaiqiang Wu, Junsong Wang*, Xueli Zhang*, Changhao Bi*. Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool. Biochemical Engineering Journal. 2019, 146(15): 132-142. https://doi.org/10.1016/j.bej.2019.03.015, 中科院二区；JCR二区；IF=4.446; Cited by 11;

4、Zaiqiang Wu*. Mixed fermentation of Aspergillus niger and Candida shehatae to produce bioethanol with ionic-liquid-pretreated bagasse. 3 BIOTECH, 2019, 9(2):41. https://doi.org/10.1007/s13205-019-1570-6, 中科院四区；JCR三区；IF = 2.893; Cited by 2;

荣誉与奖励

2022.01.26-2023.01.25 澳门大学 (University of Macau)  全职博士后 

2020.04.12-2021.11.30  香港城市大学 (City University of Hong Kong)  全职博士后 

2015.9-2019.11    南京理工大学  全日制学术型博士   专业：化学工程与技术 

2016.7-2019.8     中国科学院天津工业生物技术研究所          联合培养博士研究生

2011.9-2014.6     江苏科技大学 全日制学术型硕士研究生     专业：生物化学与分子生物学