曾园山
  • 单位：中山大学   中山医学院组织胚胎学教研室
  • 职称：教授
  • 职位：
  • 出生：1955.6
  • 性别：男
  • 社会职务： 中国解剖学会常务理事
  • 微博：

研究领域

曾园山教授：博士/硕士研究生导师，曾任中山医科大学基础医学院党委副书记、副院长和中山大学中山医学院分工会主席、组织胚胎学教研主任、中山大学工会副主席。现担任中国解剖学会常务理事、广东省解剖学会名誉理事长、中国神经科学学会神经干细胞和组织工程分会副主任、中山大学脊髓损伤研究所副所长、中山大学干细胞与组织工程教育部重点实验室副主任、广东省脑功能与脑疾病重点实验室副主任、广东省人体生物组织工程学会副理事长、广东省细胞生物学会副理事长和解剖学研究副主编、享受国务院政府特殊津贴专家。现在研究方向是：组织工程神经网络组织修复全横断脊髓损伤。在北京香山科学会议(第609次)上首次提出组织工程神经元中继器修复脊髓损伤假说，并获得国家自然科学基金重大项目课题、国家自然科学基金重点项目、国家重点研发计划项目课题、国家自然科学基金联合基金项目、国家自然科学基金面上项目等经费资助研究。至今为止，在Advanced Science、Advanced FunctionalMaterials、Biomaterials、Journalof Neuroscience等国际学术期刊上发表学术论文65篇。以第1完成人获国家卫生部科技进步奖三等奖(1998年度)和广东省自然科学奖二等奖(2017年度)。获得国家发明专利授权4项和实用新型专利授权2项。

论文与著作

1.        Lai BQ, Che MT, Feng B, Bai YR, Li G, Ma YH, Wang LJ, Huang MY, WangYQ, Jiang B, Ding Y, ZengX*, Zeng YS*. Tissue-engineered neural network graft relays excitatory signal in thecompletely transected canine spinal cord. Advanced Science, 2019, 6(22):1901240 (1 of 19) (IF: 15.804)

2.        Huang LJ, Li G, Ding Y, Sun JH, Wu TT, Zhao W, Zeng YS*. LINGO-1 deficiency promotes nerve regeneration through reduction ofcell apoptosis, inflammation, and glial scar after spinal cord injury in mice.Exp Neurol, 2019, 320:112965 (IF: 4.483)

3.        徐昊禹，邓青文，丁英，曾园山*。神经营养素-3能够促进体外受损伤大鼠脊髓神经元的存活及其神经突起生长。解剖学研究，2019，41(1):1-4

4.        Jin H, Zhang YT, Yang Y, Wen LY, Wang JH, Xu HY, Lai BQ, Feng B,Che MT, Qiu XC, Li ZL, Wang LJ, Ruan JW, Jiang B, Zeng X, Deng QW, Li G, Ding Y*, Zeng YS*. Electroacupuncture facilitates the integration of neuralstem cell-derived neural network with transected rat spinal cord. Stem CellReports, 2019, 2:274-289 (IF: 6.537)

5.        WuGH, ShiHJ, CheMT, HuangMY, WeiQS, FengB, MaYH, WangLJ, JiangB, Wang YQ, HanI, LingEA, Zeng X*, Zeng YS*. Recovery of paralyzed limb motor function in canine with completespinal cord injury following implantation of MSC-derived neural network tissue. Biomaterials, 2018, 181:15-34 (IF: 10.082)

6.        Lai BQ, Feng B, Che MT, Wang LJ, Cai S, Huang MY, Gu HY, Jiang B, Ling EA, Li M, Zeng X*, Zeng YS*. Amodular assembly of spinal cord-like tissue endows targeted tissue repair inthe transected spinal cord. Advanced Science, 2018, 5(9):1800261(1 of 14) (IF: 15.804)

7.        Li G, Che MT, Zeng X, Qiu CX, Feng B, Lai BQ, Shen HY, Ling EA, Zeng YS*. Neurotrophin-3released from implant of tissue-engineered fibroin scaffolds inhibitsinflammation, enhances nerve fiber regeneration and improves motor function incanine spinal cord injury. J Biomed Mater Res A,2018, 106(8):2158-2170. (IF: 3.231)

8.        Ma YH, Zeng X*,Qiu XC, Wei QS, Che MT, Ding Y, Liu Z, Wu GH, Sun JH, Pang M, Rong LM, Liu B,Aljuboori Z, Han I, Ling EA, Zeng YS*.Perineurium-like sheath derived from long-term surviving mesenchymal stem cellsconfers nerve protection to the injured spinal cord. Biomaterials, 2018, 160:37-55(IF: 8.806)

9.        Zou JL, Liu S, Sun JH, Yang WH, Xu YW, Rao ZL, Jiang B, ZhuQT, Liu XL, Wu JL, Chang C, Mao HQ, Ling EA, Quan DP*, Zeng YS*.Peripheral nerve-derived matrix hydrogel promotes remyelination and inhibitssynapse formation. Advanced Functional Materials, 2018, 28(13):1705739 (1 of12) (IF: 15.621)

10.     杨扬，徐昊禹，丁英，曾园山*。两种督脉电针对大鼠脊髓受损伤神经元存活的影响。解剖学研究，2019，40(1):2-5

11.     Wang JM, Qu ZQ,Wu JL, Chung P, Zeng YS*. Mitochondrial protectiveand anti-apoptosis effects of Rhodiola Crenulata extract on hippocampal neuronsin a rat model of Alzheimer’s disease. Neural Regen Res, 2017, 12(12):2025-2034 (IF: 1.769)

12.    Zhang YT, Jin H, Wang JH, Wen LY, Yang Y, RuanJW, Zhang SX, Ling EA, Ding Y*, Zeng YS*. Tail nerve electrical stimulation andelectro-acupuncture can protect spinal motor neuronsand alleviate muscle atrophy after spinal cord transection in rats. NeuralPlast, 2017, 2017:7351238 (1 of11). doi: 10.1155. (IF: 3.054)

13.     YangXH, Ding Y, Li W, Zhang RY, Wu JL, Ling EA, Wu W, Zeng YS*.Effects of electroacupuncture and the RXR signaling pathwayon oligodendrocyte differentiation in the demyelinated spinal cord of rats. Acupunct Med, 2017, 35(2):122-132(IF: 2.156)

14.     Lai BQ, Che MT,Du BL, Zeng X, Ma YM, Feng B, Qiu XC, Zhang K, Liu S,Shen HY, Wu JL, Ling EA, Zeng YS*. Transplantationof tissue engineering neural network and formation of neuronal relay into thetransected rat spinal cord. Biomaterials, 2016, 109:40-54(IF: 8.557)

15.     LinXY, Lai BQ, Zeng X, Che MT, Ling EA, Wu W, ZengYS*. Cell transplantation and neuroengineeringapproach for spinal cord injury treatment: A summary of current laboratoryfindings and review of literature (an invitedreview). Cell Transplant, 2016, 25(8):1425-1438 (IF: 3.472)

16.    Zeng X, Ma YH, ChenYF, Qiu XC, Wu JL, Ling EA, Zeng YS*. Autocrinefibronectin from differentiating mesenchymal stem cells induces the neuriteelongation in vitro and promotes nerve fiber regeneration in transected spinalcord injury. J Biomed Mater Res A, 2016, 104(8):1902-1911 (IF: 3.263)

17.     Li G, Che MT, Zhang K, Qin LN, Zhang YT, Chen RQ, Rong LM, Liu S, Ding Y, Shen HY, Long SM, Wu JL, Ling EA, Zeng YS*. Graft of theNT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration,attenuates inflammation, and induces cell migration in rat and canine withspinal cord injury. Biomaterials, 2016, 83:233-248(IF: 8.557)

18.     Lai BQ, Qiu XC,Zhang K, Zhang RY, Jin H, Li G, Shen HY, Wu JL, Ling EA, Zeng YS*. Cholera toxin B subunit shows transneuronal tracing afterinjection in an injured sciatic nerve. PLoS One, 2015, 10(12):e0144030 (1 of 13)(IF:3.234)

19.     Qiu XC, Jin H, Zhang RY, Ding Y, Zeng X, Lai BQ, Ling EA,Wu JL, Zeng YS*. Donor mesenchymal stem cell-derived neural-like cellstransdifferentiate into myelin-forming cells and promote axon regeneration inrat spinal cord transection. Stem Cell Res Ther, 2015,6(1):105 (1 of 17)(IF: 3.368)

20.     Zou J, Hu B, Arpag S, Yan Q, Hamilton A, Zeng YS, Vanoye CG, Li J. Reactivation of lysosomal Ca2+ efflux rescuesabnormal lysosomal storage in FIG4-deficient cells. J Neurosci, 2015,35(17):6801-6812(IF: 6.344)

21.     Ding Y, Zhang RY,He B, Liu Z, Zhang K, Ruan JW*, Ling EA, WuJL, Zeng YS*. Combination of electroacupuncture and grafted mesenchymalstem cellsoverexpressing TrkC improves remyelination and function in demyelinated spinal cord of rats.Scientific Reports, 2015, 5:9133 (1 of 14) (IF: 5.578)

22.     Zeng X, Qiu XC, Ma YH, Duan JJ, Chen YF, Gu HY, Wang JM,Ling EA, Wu JL, Wu WT, Zeng YS*. Integration of donor mesenchymal stemcell-derived neuron-like cells into host neural network after rat spinal cordtransection. Biomaterials, 2015, 53:184-201 (IF: 8.557)

23.     DuBL, Zeng X, Ma YH, Lai BQ, Wang JM, Ling EA, Wu JL, Zeng YS*. Graft of the gelatin sponge scaffold containing geneticallymodified neural stem cells promotes cell differentiation, axon regeneration andfunctional recovery in rat with spinal cord transection. J Biomed Mater Res A, 2015, 103(4):1533-1545(IF: 3.369)

24.     Liu Z, He B, Zhang RY, Zhang K, Ding Y, Ruan JW, Ling EA, Wu JL, Zeng YS*. Electroacupuncturepromotes the differentiation of transplanted bone marrow mesenchymal stem cellspre-induced with neurotrophin-3 and retinoic acid into oligodendrocyte-likecells in demyelinated spinal cord of rats. Cell Transplant,2015, 24(7):1265-1281 (IF:3.127)

25.     Guo JS*, Qian CH,Ling EA, Zeng YS*. Nanofiber scaffolds for treatment of spinal cord injury. Curr Med Chem, 2014, 21(37):4282-4289 (IF: 3. 715)

26.     Zhang K, Liu Z, Li G, Lai BQ, Qin LN, DingY, Ruan JW, Zhang SX, ZengYS*. Electro-acupuncturepromotes the survival and differentiation of transplanted bone marrowmesenchymal stem cells pre-induced with neurotrophin-3 and retinoic acid ingelatin sponge scaffold after rat spinal cord transection. Stem Cell Rev Rep,2014, 10(4):612-625( IF: 3.124 )

27.     DuBL, Zeng CG, Zhang W, Quan DP, Ling EA, ZengYS*. A comparative study of gelatinsponge scaffolds and PLGA scaffolds transplanted to completely transected spinal cord of rat. J Biomed MaterRes A, 2014, 102(6):1715-1725 (IF:2.834)

28.     LaiBQ, Wang JM, Ling EA, Wu JL, Zeng YS*.Graft of a tissue engineered neuralscaffold serves as a promising strategy to restore myelination after rat spinalcord transection. Stem Cells Dev, 2014, 23(8):910-921 ( IF: 4.670)

29.     Chen YF, Zeng X, Zhang K, LaiBQ, Ling EA, Zeng YS*. Neurotrophin-3 stimulates migration ofmesenchymal stem cells overexpressing TrkC. Curr Med Chem, 2013, 20(24): 3022-3033(IF: 4.070)

30.     LaiBQ, Wang JW, Duan JJ, ChenYF, Gu HY, Ling EA, Wu JL, Zeng YS*.The integration of NSC-derived and host neural networks after rat spinal cordtransection. Biomaterials, 2013, 34(12):2888-2901 (IF: 7.604)

31.     Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, HuangSF, Zhang YJ, Wu JL, FisherD, Dong H*, ZengYS*.Electroacupuncture promotes the differentiation of transplanted bone marrowmesenchymal stem cells overexpressing TrkC into neuron-like cells in transectedspinal cord of rats.CellTransplant, 2013, 22(1):65-86 (IF: 4.422)

32.     张可，曾园山，秦丽娜，刘洲，丁英，阮经文。督脉电针对早期受损伤的脊髓神经营养素-3表达的影响。解剖学研究，2012，34(6):411-415

33.     LiWJ, Li SM, Ding Y, He B, Keegan J, Dong H, Ruan JW*, Zeng YS*. Electro-acupuncture upregulates CGRP expressionafter rat spinal cord transection.NeurochemInt, 2012, 61(8):1397-1403 (IF: 2.857)

34.     ZhouY, Qu ZQ, Zeng YS*, Lin YK, Li Y, ChungP, Wong R, Hägg U. Neuroprotective effect of preadministration with Ganodermalucidum spore on rat hippocampus. Exp Toxicol Pathol, 2012, 64:673-680(IF:2.283)

35.     ZhangYQ, He LM, Xing B, Zeng X, Zeng CG, Zhang W, Quan DP, Zeng YS*. Neurotrophin-3 gene modified Schwann cells promote TrkCgene modified mesenchymal stem cells to differentiate into neuron-like cells inPLGA multiple-channel conduit. Cells Tissues Organs,2012, 199(4):313-322 (IF: 2.302)

36.     Ma YH, Zeng X, Zhang K, Zeng YS*.A new in vitro injury model of mouseneuronsinduced by mechanical scratching. Neurosci Lett, 2012, 510(1):14-19 (IF: 2.055)

37.     ZhangYJ, Zhang W, Lin CG, Ding Y, Huang SF, Wu JL,Li Y, Dong H, Zeng YS*. Neurotrophin-3 gene modified mesenchymal stemcells promote remyelination and functional recovery in the demyelinated spinalcord of rats. J Neurol Sci, 2012, 313(1-2):64-74 (IF: 2.324)

38.     Qu ZQ, Zhou Y, Zeng YS*, Lin YK, Li Y, Zhong ZQ, Chan WY*. Protectiveeffects of a rhodiola crenulata extract and salidroside on hippocampalneurogenesis against streptozotocin-induced neural injury in the rat. PLoS One,2012, 7(1):e29641 (IF: 4.411)

39.     GuoJS, Ma YH, Yan Q, Wang LL, Zeng YS*, Wu JL, Li Jun*.Fig4 expression in the nervous system and its protection against abnormallysosomal accumulation. J Neuropath Exp Neurol, 2012, 71(1):28-39 (IF: 4.564)

40.     ZengX, Zeng YS*, Ma YH, LuLY, Du BL, Zhang W, Li Y, Chan WY*. Bone marrow mesenchymal stem cellsin a three dimensional gelatin sponge scaffold attenuate inflammation, promoteangiogenesis and reduce cavity formation in experimental spinal cord injury. CellTransplant, 2011, 20(11/12):1881-1899 (IF: 6.204)

41.     陈元峰，曾湘，曾园山*。腺病毒介导的神经营养素-3基因能够在大鼠脊髓前角运动神经元内过表达神经营养素-3。解剖学研究，2011，33(6):405-408

42.     Liu Z, Ding Y, Zeng YS*. A new combined therapeutic strategy of GovernorVessel electro-acupuncture and adult stem cell transplantation promotes therecovery of injured spinal cord (an invited review). Curr Med Chem, 2011, 18:5165-5171(IF: 4.630)

43.     王俊梅，曾园山*。过表达NT-3-SCs和过表达TrkC-NSCs移植减少脊髓损伤处瘢痕形成和促进神经再生。解剖学研究，2011，33(5):367-372

44.     Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, HuangSF, Zhang YJ, Wang S, DongH, ZengYS*. Bone marrow mesenchymal stem cells and electro-acupuncturedownregulate the inhibitor molecules and promote the axonal regeneration in thetransected spinal cord of rats. Cell Transplant,2011, 20(4):475-491(IF:6.204)

45.     Wang JM, Zeng YS*, Wu JL, Li Yan, Teng YD*. Cograft of Schwann cells and neural stem cellsoverexpressing neurotrophin-3 and TrkC respectively after rat spinal cordtransection. Biomaterials, 2011, 32(30):7454-7468 (IF: 7.882)

46.     冯英，曾园山*。在组织学与胚胎学课程中应用TBL教学模式的初步探索。中国组织化学与细胞化学杂志，2011，29(4)：377-379

47.     卢丽雅，张巍，曾园山*，曾湘，何兵，黄斯凡，李燕。神经营养素-3基因修饰骨髓间充质干细胞的明胶海绵圆柱体支架移植促进大鼠全横断脊髓损伤部分结构和功能修复。中国康复医学杂志, 2011, 26(7):603-608

48.     DuBL, Xiong Y, Zeng CG, He LM, Zhang W, Quan DP, Wu JL, Li Y, Zeng YS*. Transplantation of artificialneural construct partly improved spinal tissue repair and functional recoveryin rats with spinal cord transection. Brain Res, 2011, 1400:87-98(IF:2.463)

49.     Huang SF, Ding Y, Ruan JW, Zhang W, Wu JL, He B,Zhang YJ, Li Y, Zeng YS*. An experimentalelectro-acupuncture study in treatment of the rat demyelinated spinal cordinjury induced by ethidium bromide. Neurosci Res,2011,70:294-304(IF:2.473)

50.     何兵，丁英，曾园山*，黄斯凡，李燕。MOG 1-125诱发的实验性自身免疫性脑脊髓炎大鼠模型。解剖学研究，2011，33(2):126-130

51.     Yan Q, Ruan JW, Ding Y, Li WJ, Li Y, ZengYS*.Electro-acupuncture promotes differentiation ofmesenchymal stem cells, regeneration of nerve fibers and partial functionalrecovery after spinal cord injury. Exp Toxicol Pathol, 2011, 63:151-156 (IF:2.283)

52.     李淑敏，李文杰，曾园山，阮经文。不同局部穴位电针对大鼠受损伤脊髓组织降钙素基因相关肽表达的影响。中国康复医学杂志，2010,25(9):823-827

53.     阮经文，李淑敏，李文杰，曾园山。艾灸与电针督脉腧穴上调大鼠受损伤脊髓组织表达降钙素基因相关肽的对比研究。中华物理医学与康复杂志，2010, 32(9):657-661

54.     ZhangW, Yan Q, Zeng YS*, Zhang XB,Xiong Y,  Wang JM,  Chen SJ, Li Y, Bruce IC, Wu WT*. Implantation of adult bone marrow-derived mesenchymal stemcells transfected with the neurotrophin-3 gene and pretreated with retinoicacid in completely transected spinal cord. Brain Res, 2010,1358:256-271 (IF: 2.463)

55.     Li WJ, Pan SQ, Zeng YS*,Su BG, Li SM, Ding Y, Li Y, Ruan JW*. Identification of acupuncture-specific proteins in the process ofelectro-acupuncture after spinal cord injury. Neurosci Res, 2010,67:307-316 (IF:2.473)

56.     ZhangYQ, Zeng X, He LM, Ding Y, Li Yan, Zeng YS*. NT-3 genemodified Schwann cells promote TrkC gene modified mesenchymal stem cells todifferentiate into neuron-like cells invitro. Anat Scie Inter, 2010, 85(2):61-67 (IF: 0.719)

57.     周春春，曾园山*，秦亚静，丁英，李燕，钟志强。缬草对慢性应激导致的抑郁大鼠大脑海马p-CREB阳性神经元数量的影响。解剖学研究，2010，32(2):81-87

 授权专利情况

       (1)曾园山, 李戈。一种具有趋化功能的生物活性支架的制备和应用。获中华人民共和国发明专利, 于2018年5月4日授权，发明专利号: ZL 201510252317.9

(2)曾园山, 李戈, 孙佳慧。一种基质化去细胞神经支架。获中华人民共和国实用新型专利, 于2018年1月30日授权，实用新型专利号: ZL201621132126.5

(3)曾园山, 曾湘。一种用于修复脊髓损伤的人工神经网络样导管的构建。获中华人民共和国发明专利, 于2015年11月6日授权，发明专利号: ZL201010506133.8

(4)曾园山, 李戈。一种具有趋化功能的生物活性支架的制备和应用。获中华人民共和国实用新型专利, 于2015年12月9日授权，实用新型专利号: ZL 201520319632.4

(5)曾园山, 曾湘。一种用于修复神经损伤的明胶海绵圆柱体支架的构建。获中华人民共和国发明专利, 于2011年6月8日授权，发明专利号: ZL 200910040176.9

(6)曾园山, 王俊梅。人神经营养素-3受体基因重组腺病毒构建方法。获中华人民共和国发明专利, 于2008年7月16日授权，发明专利号: ZL 200510033569.9

主编教材:

2001年主编《人体结构学》, 94.7万字, 北京: 科学出版社出版;

2001年主编《组织学胚胎学(考试辅导丛书)》, 33万字, 北京: 科技文献出版社出版;

2002年主编《组织学与胚胎学实验指导》, 20万字, 北京: 科技文献出版社出版;

2004年主编《组织学胚胎学(考试辅导教材)》修订版, 47.7万字, 北京: 科技文献出版社出版;

2004年主编《组织学和胚胎学(专科生用教材)》, 30万字, 北京: 科学出版社出版;

2006年主编《组织学胚胎学考点》, 37.6万字, 北京: 科技文献出版社出版;

2009年主编《组织学与胚胎学导学与应试指南》, 30.9万字, 北京: 科技文献出版社出版;

2010年主编全国医药高等学校规划教材《组织学和胚胎学》, 25万字, 北京: 科学出版社出版;

2011年主编《组织学与胚胎学实验指导》, 11.5万字, 北京: 中国医药科技出版社出版;

2012年主编《ALaboratory Manual of Histology and Embryology, 组织学与胚胎学实验指导(英文版)》,19.4万字, 北京: 人民卫生出版社出版。

2014年主编《组织学与胚胎学实验指导》, 14.6万字, 北京: 人民卫生出版社出版。

2015年主编临床医学数字化规划教材《组织学与胚胎学》, 60.5万字, 北京: 人民军医出版社。

2018年作主编5年制本科生《组织学与胚胎学(第9版全国规划教材)》, 55万字, 北京: 人民卫生出版社出版。

2018年作为主编5年制本科生《组织学与胚胎学学习指导及习题集》, 36.7万字, 北京: 人民卫生出版社出版。2018年12月出版



荣誉与奖励

2017年，曾园山、丁英、阮经文、刘洲、李文杰、张可、陈雅云、曾湘、李晓滨、李淑敏，“督脉经穴电针通过NT-3介导移植的成体干细胞修复脊髓损伤的研究”，获广东省自然科学奖二等奖。 证书号:A04-2-01-R01;奖励日期: 2018年2月; 排名:第1获奖人。

2001年，曾园山、张媛媛、章尧、顾熊飞，“脊髓可塑性变化的神经营养活性物质系列研究”，获广东省自然科学优秀学术论文奖二等奖。 证书号:022; 奖励日期:2001年12月; 排名:第1获奖人。

1998年，曾园山、吴良芳、郭畹华、章尧、罗超权，“吗啡促进脊髓可塑性变化的研究”，获国家卫生部科技进步奖三等奖。证书号:98303801; 奖励日期:1998年9月; 排名:第1获奖人。

1998年，曾园山、吴良芳、郭畹华、章尧、罗超权，“吗啡促进脊髓可塑性变化的研究”，获广东省医药卫生科技进步奖三等奖。 证书号:粤卫科字(98)第139号; 奖励日期:1998年7月; 排名:第1获奖人。

1996年，曾园山、吴良芳、保天然、廖德阳、李秀琼，“吗啡促进脊髓损伤修复的研究”,获成都市科技进步奖三等奖。 证书号:963186; 奖励日期:1996年1月; 排名:第1获奖人。

1996年，曾园山、郭畹华、黄巨恩，“胰岛素促进移植在缺损运动神经元脊髓中异体脊髓组织的存活和生长”，获广东省自然科学优秀学术论文奖三等奖。 证书号:91; 奖励日期:1996年10月; 排名:第1获奖人。

1995年，曾园山、郭畹华、黄巨恩，“胰岛素对移植的胚胎脊髓组织存活与生长的影响”，获中山医科大学优秀论文奖一等奖。 证书号:无; 奖励日期:1995年5月; 排名:第1获奖人。

1994年，曾园山、吴良芳，“吗啡对大鼠脊髓可塑性影响的形态学研究”，获广东省自然科学优秀学术论文奖三等奖。证书号:86; 奖励日期:1994年8月; 排名:第1获奖人。