DNA重组技术的基本原理及医学应用Basic Principles and Medical Application of Recombinant DNA Technology
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2 牛刀小试
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尚丹丹
目录
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1 绪论 Introduction
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1.1 教学计划
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1.2 绪论 Introduction
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2 蛋白质结构与功能 Structure and Function of Proteins
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2.1 氨基酸的结构 Structure of amino acids
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2.2 氨基酸的分类与性质 Classification and characterization of amino acids
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2.3 蛋白质的分子结构 Molecular Structure of proteins
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2.4 蛋白质结构与功能的关系 Relationship on structure and function of proteins
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2.5 蛋白质的理化性质 The Physical and Chemical Characters of Proteins
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2.6 蛋白质的变性 Denaturation of proteins
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3 核酸结构与功能 Structure and Function of Nucleic acid
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3.1 核酸的组成与一级结构 Molecular compositions and primary structure of nucleic acids
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3.2 DNA的双螺旋结构 DNA double helix
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3.3 DNA的超螺旋结构 The superhelix of DNA
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3.4 RNA的空间结构 The spatial structure of RNA
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3.5 核酸的理化性质 Physical and chemical properties of nucleic acids
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4 酶与酶促反应 Enzyme and Enzymatic Reaction
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4.1 酶的分子结构 Properties of enzymes
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4.2 酶促反应动力学 Kinetics of enzymatic reaction
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4.3 酶的调节 Regulation of enzymes
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4.4 酶在医学中的应用 Application of Enzymes in Medicine
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5 糖代谢 Carbohydrate Metabolism
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5.1 糖的无氧氧化 Anerobic oxidation of glucose
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5.2 红细胞代谢 Erythrocyte metabolism
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5.3 糖酵解的调节 Regulation of glycolysis
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5.4 糖的有氧氧化 Tricarboxylic acid cycle
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5.5 磷酸戊糖途径 Pentose Phosphate Pathway
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5.6 糖原的合成与分解 Glycogen metabolism
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5.7 糖异生 Gluconeogenesis
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5.8 血糖及其调节 Blood glucose and its regulation
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6 生物氧化 Biological Oxidation
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6.1 生物氧化的特点和呼吸链 Characteristics of biological oxidation and respiratory chain
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6.2 神奇的能量货币 ATP The amazing energy currency：ATP
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6.3 氧化磷酸化的偶联机制 Coupling mechanism of oxidative phosphorylation
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6.4 氧化磷酸化的影响因素 Factors Affecting Oxidative Phosphorylation
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7 脂质代谢 Lipids Metabolism
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7.1 甘油三酯的分解代谢 Catabolism of triglyceride
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7.2 酮体的生成和利用 Production and uses of ketone bodies
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7.3 甘油三酯的合成 Anabolism of triglycerides
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7.4 胆固醇代谢 Metabolism of cholesterol
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7.5 血浆脂蛋白代谢 Metabolism of plasma lipoproteins
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8 蛋白质的消化吸收和氨基酸代谢 Amino Acid Metabolism
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8.1 蛋白质的消化吸收与腐败 Digestion, absorption, and putrefaction of proteins
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8.2 转氨基作用与脱氨基 Amino acid transamination
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8.3 氨的转运 Ammonia transport
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8.4 血氨的来源和去路 Source and route of blood ammonia
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8.5 尿素循环和高血氨症 Urea cycle and hyperammonemia
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8.6 一碳单位 One carbon unit
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8.7 甲硫氨酸循环 Methionine cycle
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8.8 芳香族氨基酸的代谢 Metabolism of Aromatic Amino Acids
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9 核苷酸代谢 Nucleotide Metabolism
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9.1 嘌呤核苷酸代谢 Metabolism of purine nucleotides
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9.2 嘧啶核苷酸代谢 Metabolism of pyrimidine nucleotides
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9.3 核苷酸抗代谢物 Nucleotide Antimetabolites
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10 DNA的合成 DNA Biosynthesis
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10.1 复制的规律与复制体系 Systems of DNA replication
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10.2 原核生物DNA复制过程 DNA replication process of prokaryote
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10.3 真核生物DNA复制过程 DNA replication in prokaryotes
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10.4 端粒和端粒酶 Telomere and telomerase
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10.5 逆转录 Reverse transcription
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10.6 DNA损伤与修复 DNA Damage and Repair
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11 RNA的合成 RNA Biosynthesis
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11.1 原核生物转录的模板和酶 Template and enzyme of prokaryotic RNA transcription
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11.2 原核生物转录的过程 Transcription process of prokaryote
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11.3 真核生物转录的过程 Transcription process of eukaryote
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11.4 真核生物前体RNA的加工 Eukaryotic pre-RNA processing
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12 蛋白质的合成 Protein Biosynthesis
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12.1 蛋白质的合成体系 Systems of protein synthesis
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12.2 蛋白质的生物合成过程及靶向输送
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12.3 蛋白质合成的干扰和抑制 Interference and inhibition of protein synthesis
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13 基因表达调控 Regulation of Gene Expression
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13.1 基因表达调控的基本概念与特点 Concept and characterization of procaryotic gene expression regulation
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13.2 原核生物基因表达调控-操纵子模型 Regullation of gene expression in prokaryotes-Operon model
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13.3 真核生物基因表达调控 Regulation of gene expression in eukaryotes
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14 细胞通讯与细胞信号转导 Cell Communication and Celluar Signal Transduction
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14.1 细胞外信号与受体 Extracellular signals and receptors
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14.2 第二信使 The second messenger
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14.3 cAMP-PKA途径 cAMP-PKA signal pathway
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14.4 Ras/MAPK信号转导途径 Ras/MAPK signal pathway
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15 癌基因和抑癌基因 Oncogenes and tumor suppressor genes
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15.1 癌基因 Oncogene
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15.2 抑癌基因 Tumor suppressor gene
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16 常用分子生物学技术原理 Molecular Biology Techniques
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16.1 分子杂交与印迹技术 Molecular Hybridization and Blotting Technology
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16.2 PCR技术 PCR technology
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16.3 重组DNA技术的工具酶与载体Enzymes and Vectors
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16.4 DNA重组技术的基本原理及医学应用Basic Principles and Medical Application of Recombinant DNA Technology
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17 临床问题专辑
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17.1 夜盲症与维生素A
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17.2 骨质疏松的诊断与治疗
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17.3 认识胰岛素
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17.4 嘌呤核苷酸的分解代谢与痛风
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18 生物化学与分子生物学实验指导
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18.1 蛋白质的定量测定:酚试剂法（改良Lowry法）
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18.2 理化因素对唾液淀粉酶活性的影响
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18.3 激素对血糖浓度的影响
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18.4 肌肉组织氨基移换作用
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18.5 血清丙氨酸氨基转移酶活性测定
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18.6 分子生物学实验指导
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