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Effect of insulin and hypoglycemia
Rescue of insulin-induced hypoglycemia
Two mice were fasted for 12-20h
Mouse1（weight 20g）--inject insulin(0.5－1ml，intraperitoneal injection) ---16mins later, observe symptoms of hypoglycemic shock: progressive nervous irritability that leads to anxiety, seiures, and even coma---rescue with 50%glucose(0.5-1ml，intraperitoneal injection)--- behavior: normal(active)
Mouse2（weight 20g）--inject normal saline(the same volume，intraperitoneal injection)---30mins later, behavior: active
Purpose:
1. Observe the response of hypoglycemia caused by excessive insulin
2. To understand the rescue methods of hypoglycemia
Principle:
Insulin is a protein hormone secreted by islet beta cells. Its main physiological function is to regulate metabolism. Insulin is the only hormone in the body that reduces blood sugar and promotes the synthesis of glycogen, fat and protein. After injecting a large amount of insulin into mice, it can reduce blood glucose and cause hypoglycemia. In the early stage, it can show symptoms such as hunger, rapid heartbeat, anxiety, tremor and convulsion. In severe cases, it can cause coma, shock, brain injury and even death. In order to prevent the serious consequences of hypoglycemia, people can drink sugar water or eat. In severe cases, 50% glucose should be injected intravenously immediately to supplement blood glucose to the normal level.
