Personal introduction
Microbial Experiment Technology

Main Teacher:张小华

Teacher team:6in total

  • Zhang Xiaohua
  • Cao Qingguo
  • Xu Jianmin
  • Liu Yan
  • Lu Jianlan
  • Liu Li
College: Jiangsu Vocational College of Agriculture and Forestry
Curriculum: Biotechnology
English Name: Microbial Experiment Technology
Credit: 3
Period: 46
Course description
"Microbial Experimental Technology" is an experimental professional basic course, students need to have a strong hands-on ability. The course takes the combination of work and study as the starting point, based on the needs of microbiological testing, uses the testing technology and working process as the carrier to design the learning situation, integrates the software and hardware conditions in the internal and external training, and closely connects with the actual production, and conducts integrated teaching mode of "teaching, learning, and acting". It includes two parts, basic operation technology and comprehensive detection technology, covering the basic operation of microorganisms. Through the study of this course, students should master the basic operations of microbes and independently complete microbial detection experiments. This course is conducive to cultivate students' rigorous scientific attitude and ability to analyze and solve problems.
Teaching team

Zhang Xiaohua

Professional title:associate professor

Cao Qingguo

Professional title:associate professor

Xu Jianmin

Professional title:associate professor

Liu Yan

Professional title:教师

Lu Jianlan

Professional title:教师

Liu Li

Professional title:教师

Syllabus


Teaching design

(1)Teaching model design

Thiscourse aims to change the traditional teaching mode based on "teaching"to the teaching mode based on "learning"and strives to achieve threegoals:

1.Integration: Aiming at the integration of knowledge, theory and practice, and  integration of teaching-learning-acting, anintegrated teaching model combining classrooms and practical training.

2.Process-oriented work: It aims to establish a process-oriented teaching modelthat takes job requirements as the mainstay, work flow as the main line, andpractical teaching as the main body.

3.Operation standardization: Training is carried out completely in standardizedoperations, so that skill training and skill assessment are perfectly combined.

(2)Teaching content design

Thiscourse starts from the requirements of microbiological testing and applicationpost competence. After enterprise research, it is determined that there arethree posts related to the course, which are bacterial cultivation, productionand testing positions. Strain cultivation positions require staff to master theskills of medium preparation, aseptic operation, separation culture, andmicroscopic identification; production positions require staff to master mediumpreparation, microbial cultivation, disinfection and sterilization, bacterialgrowth skills and identification of the situation; testing positions requireemployees to master the skills of three basic aspects of microbial operationtechnology, pathogen identification, and issuing test reports. This courseselects the following contents for teaching based on job skills.

Table 1 "Microbial ExperimentalTechnology" course schedule

Serial  number

Experiment name

Hours

1

Application of microscope

2

2

Gram staining technique

2

3

Morphological observation technique  of fungi

4

4

Colony identification technique  of common microbes

2

5

Binding technique of glassware

2

6

Preparation technique of culture  medium

4

7

Use of autoclave

4

8

Aseptic operation technology

2

9

Detection technology of colony  count

6

10

Coliform detection technology

6

11

Determination of growth curve

4

12

Direct microscopic counts with  a hemocytometer

4

13

Microbial fermentation food  technology

4

Total

46


Teaching conditions

(1)Practice conditions on campus

Thewhole area of Jiangsu Agriculture and Forestry Vocational and Technical Collegeis more than 2000 square meters. There are 120 microbiology laboratories. Theinstrument assets of the laboratory are valued more than 3.6 million yuan. Thelaboratory is equipped with 3 microscope imaging systems, 160 ordinary opticalmicroscopes, 20 ultra-clean worktables, 10 high-pressure steam sterilizers, allof which can meet the teaching task of this course.

(2)Off-campus practice base

Off-campuspractice bases include Jurong City Disease Prevention and Control Center,Jurong City Sewage Treatment Center, Jurong Dongfangzi Mulberry Wine Co., Ltd.

(3)Network teaching environment

Teachersuse the Superstar Panya online teaching platform to perform teaching tasks, anduse PPT courseware, teaching videos, 3D animation, reference materials andother resources to assist teaching. Classes are carried out with teacherdemonstrations, student-controlled video exercises, and teacher re-instruction,interspersed with student discussions and teacher analysis of important anddifficult points.


Teaching methods

Theteaching methods used in this course are mainly the following four types,including multimedia teaching method, physical demonstration method,demonstration guidance method, and real-world practice teaching method.

①Multimediateaching method

Multimediateaching courseware and 3D animation are used to increase the related information,so that students can have a more comprehensive understanding on the form ofmicrobes and the use of instruments, and teachers may use dynamic imageprojections to make up for the lack of practical classes and help studentsincrease their perceptual knowledge. For example, teachers use 3D animation toshow the structure of the microscope.

②Physicaldemonstration method

Thephysical demonstration can not only help students operate and visualize theoperation of the equipment, but also allow students have a more intuitiveimpression on different colony forms, helping students easily master thelearning items. For example, students can observe colony morphology.

③Model guidance method

Inthe teaching process, the teacher first demonstrates a single skill item on thespot, and the students repeat the operation and continue to practice. Theteacher guides the students and points out their mistakes, so that the studentsrealize standardized operations. For example, the use of microscopes.

④Teachingmethod of reality drill

Teacherscontinue to teach in the enterprise. According to the task of the projectdesign, the on-site teacher first demonstrates, and then the students practicethe operation to master the operation essentials to achieve the teachingconcept of "learning in operation, operating in learning". Forexample, the detection technology of coliform bacteria.


Assessment and evaluation

(1)Assessment method

Thiscourse adopts the "results and processes" method to carry out theassessment. The specific implementation is as follows:

Theassessment mode is divided into ordinary assessment and centralized assessment,with a score ratio of 6: 4. Among them, the ordinary assessment consists ofattendance, questioning, homework, practical training performance, and experimentalreport. The ratio is 2: 2: 2: 2: 2. The assessment is carried out in the formof final skill assessment.

(2)Evaluation method

Atthe end of each semester, students will evaluate the performance of theteachers, teaching evaluation indicators include the following 10 aspects:teachers' morality, teaching attitude, teaching and education, teachingcontent, teaching methods, extracurricular tutoring, homework correction,examination and examination, experimental training, teaching ability, studentswill objectively evaluate teachers' teaching level.

The teaching supervision group from the second-level college where thecourse is located organizes the teaching information officer and relevant classstudents to investigate the teaching behavior of the instructors in eachsemester and provides a comprehensive evaluation to the teachers.

Course Evaluation

Teaching Resources
Chapters | File type   | Modified time | Size | Remark
1.1 Experiment 1: Application of Microscope
作业
.work
2020-05-17 --
 
文档
.docx
2020-05-17 733.06KB
 
文档
.pdf
2020-05-18 4.44MB
 
视频
.mp4
2020-05-18 63.49MB
 
文档
.pptx
2020-05-19 2.49MB
 
视频
.mp4
2020-05-19 33.48MB
 
视频
.mp4
2020-05-24 41.96MB
1.2 Experiment 2:  Gram Staining Technique
文档
.docx
2020-05-14 1.85MB
 
文档
.pdf
2020-05-14 4.44MB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 2.29MB
 
视频
.mp4
2020-05-19 930.64MB
 
视频
.mp4
2020-05-19 23.44MB
 
视频
.mp4
2020-05-24 43.23MB
1.3 Experiment 3 :Morphological observation technique of fungi
文档
.pdf
2020-05-17 1.35MB
 
文档
.pdf
2020-05-17 1.39MB
 
文档
.pdf
2020-05-17 1.17MB
 
作业
.work
2020-05-19 --
 
文档
.pdf
2020-05-26 91.46KB
 
文档
.pdf
2020-05-26 125.00KB
 
文档
.pptx
2020-05-28 2.44MB
 
文档
.pptx
2020-05-28 1019.50KB
 
视频
.mp4
2020-05-28 61.92MB
 
视频
.mp4
2020-06-15 46.24MB
 
视频
.mp4
2020-06-15 14.30MB
1.4 Experiment4:Colony Identification Technique of Common Microbes
文档
.pdf
2020-05-14 229.78KB
 
文档
.pdf
2020-05-17 1.26MB
 
作业
.work
2020-05-18 --
 
文档
.pptx
2020-05-19 831.68KB
 
视频
.mp4
2020-05-21 24.02MB
 
视频
.mp4
2020-05-27 52.16MB
1.5 Experiment5:Binding Technique of Glassware
作业
.work
2020-05-17 --
 
文档
.pdf
2020-05-19 295.21KB
 
视频
.mp4
2020-05-21 57.18MB
 
视频
.mp4
2020-05-21 40.89MB
 
文档
.pdf
2020-05-29 680.79KB
 
视频
.mp4
2020-05-29 28.64MB
1.6 Experiment6:Preparation technique of Culture Medium
作业
.work
2020-05-17 --
 
文档
.pdf
2020-05-17 220.57KB
 
文档
.pdf
2020-05-17 152.27KB
 
文档
.pptx
2020-05-19 761.04KB
 
视频
.mp4
2020-05-19 17.70MB
 
视频
.mp4
2020-05-20 53.50MB
 
视频
.mp4
2020-05-26 41.72MB
1.7 Experiment7:The Use of Autoclave
文档
.pptx
2020-05-07 703.41KB
 
文档
.pdf
2020-05-07 221.32KB
 
文档
.doc
2020-05-07 45.50KB
 
文档
.docx
2020-05-14 1.42MB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 8.06MB
 
视频
.mp4
2020-05-19 9.71MB
 
视频
.mp4
2020-05-23 39.50MB
1.8 Experiment 8: Aseptic operation technology
文档
.docx
2020-05-14 17.58KB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 2.09MB
 
视频
.mp4
2020-05-19 80.15MB
 
视频
.mp4
2020-05-19 2.09MB
 
视频
.mp4
2020-05-21 92.68MB
2.1 Experiment 9:Detection technology of colony count
文档
.docx
2020-05-14 47.49KB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 3.07MB
 
视频
.mp4
2020-05-19 363.37MB
 
视频
.mp4
2020-05-21 76.95MB
 
视频
.mp4
2020-05-23 112.08MB
2.2 Experiment 10: Coliform detection technology
文档
.docx
2020-05-14 49.35KB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 5.17MB
 
视频
.mp4
2020-05-19 138.41MB
 
视频
.mp4
2020-05-19 92.56MB
 
视频
.mp4
2020-05-21 56.43MB
2.3 Experiment 11:  Determination of Growth Curve
作业
.work
2020-05-17 --
 
文档
.docx
2020-05-17 137.42KB
 
文档
.pptx
2020-05-19 1.03MB
 
视频
.mp4
2020-05-21 50.88MB
 
视频
.mp4
2020-05-23 32.67MB
2.4 Experiment 12:  Direct Microscopic Counts with a Hemocytometer
文档
.docx
2020-05-14 779.61KB
 
文档
.pdf
2020-05-14 28.11MB
 
作业
.work
2020-05-17 --
 
文档
.pptx
2020-05-19 2.01MB
 
视频
.mp4
2020-05-19 59.93MB
 
视频
.mp4
2020-05-23 25.85MB
 
视频
.mp4
2020-05-23 32.38MB
2.5 Experiment 13: Microbial fermentation food technology
文档
.docx
2020-05-01 49.75KB
 
作业
.work
2020-05-17 --
 
视频
.mp4
2020-05-18 76.81MB
 
文档
.pptx
2020-05-19 13.09MB
 
视频
.mp4
2020-05-19 181.97MB
 
视频
.mp4
2020-05-19 27.84MB
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